Pharmaceutical industries are in need of new bioactive molecules to precisely act against the newly developing infectious diseases. In view of this, an attempt was made in this study for the isolation of a potential bioactive molecule producing bacterium from the sea anemone, Heteractis aurora, collected from Mandapam, Ramanathapuram district, Tamil Nadu, India. Eleven symbiotic bacteria were totally isolated in which the isolate SAM 9 revealed promising antimicrobial activities against six clinically isolated human bacterial pathogens, namely, Proteus mirabilis, Klebsiella oxytoca, Salmonella paratyphi, Salmonella typhi, Escherichia coli, and Staphylococcus aureus with the growth inhibition activities of 54%, 49%, 47%, 43%, 39%, and 36%, respectively. The isolate SAM 9 was identified as Paenibacillus macerans using 16S rRNA molecular sequence method. During the media optimization, this isolate showed maximum bioactive molecule production at 96 h incubation with the other cultural conditions of pH 8, 35°C, maltose, and ammonium nitrate at the ratio of 2:1. Further, the bioactive molecule was purified using reversed-phase high-performance liquid chromatography and found one molecule responsible for the bioactivities. From the overall observations, the bioactive molecule produced by P. macerans SAM 9 showed the possibilities for future development as a promising therapeutical agent against many deadly infectious pathogens.
Key words: Sea anemone; bioactive molecule; antimicrobial; Growth kinetics; Paenibacillus macerans; Enhanced production
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