Interaction of Ketoconazole with Bovine Serum Albumin: Electrochemical, Spectroscopic and Molecular Modeling Studies
Babu Gowda, Mallappa M., Jayant I. Gowda, Raghavendran Rengasamy.
Abstract
ABSTRACT
The interaction of ketoconazole (KTZ) with bovine serum albumin (BSA) has been investigated by cyclic voltammetry, differential pulse voltammetry, UV-Vis absorption and fluorescence spectroscopy, viscosity measurements as well as molecular modeling methods. The measurements were performed in 0.1 mol L-1 phosphat buffer solution at pH = 7.4. Shifts in the peak potentials in cyclic Voltammetry, spectral changes in UV absorption and fluorescence titration, an increase in viscosity of BSA and the molecular modeling methods strongly support the electrostatic interaction between KTZ and BSA. The thermodynamic parameters ΔH, ΔG and ΔS at different temperatures were calculated, showing that the electrostatic interactions and hydrophobic interaction are the main forces for the binding of KTZ to BSA. The binding constant (Kb) determined by UV absorption and fluorescence measurements are very close to the value determined by cyclic Voltammetry assuming that the binding equilibrium is static. Moreover, from molecular modeling method, a docked structure with minimum energy was obtained in which KTZ was located in minor grooves of BSA.
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
We use cookies and other tracking technologies to work properly, to analyze our website traffic, and to understand where our visitors are coming from. More InfoGot It!