The objective of the study is to evaluate genomic DNA damage, locomotor, anxiety and free radical scavenging enzymes level in brain after exposure of noise stress. Noise stress was done by broadband white noise generator and Indigofera tinctoria (300 mg/Kg .b.w.) administered orally. Noise exposure could increase superoxide dismutase, lipid peroxidation, catalase, glutathione peroxidase, Nitric oxide, Glutathione S transferase and decrease reduced glutathione, Glutathione Reductase, Vitamin C, and protein thiols suggesting that oxidative imbalance in brain regions and adrenal when expose to noise. The elevated plus maze shows significant decrease in the number of entries and time spent in the open arm. In the rota rod, significant decrease in time spent in rota rod. Significant increase in time taken to cross in seconds and number of slips in narrow beam. Noise exposed brain region (Cerebellum, Hypothalamus and hippocampus) showed a significant increase in the DNA ladder pattern. This outcome of the results suggest that noise stress affect genomic DNA, biochemical, locomotor activity and anxiety disorder, in rat. However, oral administration of I.tinctoria significantly prevented noise stress induced changes. These results conclude that I.tinctoria may possess neuroprotective effects and the antioxidant property of the plant may have resulted in its therapeutic efficacy.
Key words: Oxidative stress, DNA fragmentation, Antioxidant, Motor coordination, Anxiety, Behavior
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