Background:
Antibiotic resistance is a global problem with high morbidity and mortality in healthcare institution that requires an antimicrobial alternative and ittle is known of antibacterial agents derived from insect sources.
Aim:
The aim of the study was to examined antibacterial properties of secondary metabolites from insect-associated bacteria.
Methods:
Thirty-three wasps were obtained from abandoned structures in a local Community. Bacteria were isolated and identified by Sanger sequencing method. The metabolites were extracted and antibacterial potential was tested against different pathogens. The metabolites concentration was analyzed via High Performance Liquid Chromatography (HPLC). The metabolites and their clinical potentials were explored through STRING analysis.
Results:
Fifteen bacteria were isolated and 9 exhibited antibacterial properties consisting of Enterococcus faecalis (2). Pseudomonas fragi (2), Propionibacterium spp (1), Comamonas terrigena (2), Staphylococcus galinarum (1), Levi lactobacillus brevis (1). Antibiogram revealed that the secondary metabolite had inhibitory effects against all pathogens. The HPLC results indicated that the control and metabolites were eluted at 1.288 and 1.259 mins respectively with concentration ranging from 16.68 to 4761.82 mg/l. STRING analysis showed that the metabolites were gallidermin, enterocin, propionicin, and phenazine.
Conclusion:
The study concluded that the metabolites show activity against test strains and therefore a source of novel antimicrobials for addressing the global drug-resistant pathogens.
Key words: Antibacterial Properties, Gallidermin, Secondary metabolites, Wasp-associated bacteria
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