A total of 170 pus swabs samples were collected: (60 samples from multiple abscesses in sheep, 50 from lung abscess in young calf from abattoir and 60 from metritis cattle) for isolation and identification of Corynebacterium pseudotuberculosis. Out 18 isolates from 60 samples of multiple abscesses in sheep in a percentage (30%), out 6 isolates from 50 samples of lung abscess young calf in a percentage (12 %) and finally out 10 isolates from 60 samples of metritis from cattle in a percentage (16.7%). Thirty-four isolates of Corynebacterium pseudotuberculosis isolated from different samples were tested for their sensitivity to 10 antimicrobial agents. The isolates were highly sensitive to Vancomycin (VA) Streptomycin (S), moderately sensitive to Gentamicin (CN), Erythromycin (E), Tetracycline (TE), and Chloramphenicol (C) but were resistant to Cephotaxime (CTX), Penicillin (P), Rifampicin (RD) and Amoxycillin-Clavulenic acid (AMC). 12 isolates of Corynebacterium pseudotuberculosis has no ability to form biofilm (35.3%), 5 isolates has ability to form weak biofilm (14.7%), 13 isolates has ability to form moderate biofilm formation (38.2%), finally there are 4 isolates has ability to form strong biofilm formation (11.8%). Nine isolates of Corynebacterium pseudotuberculosis were tested by PCR for detection of sigma factor; only 2 isolates (one from lymph node abscess of sheep and another from lung tissue abscess of cattle) have virulent gene (sigma factor) with size 490 bp.
Key words: Corynebacteria pseudotuberculosis, biofilm, antibiotic sensitivity, sigma factor.
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