Original Research |
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Cultivation of Trichomonas intestinalis in Robinson Media
Özlem M. Aycan, Nilgün Daldal, Ülkü Karaman, Metin Atambay . Abstract | | | |
T. intestinalis is a protozoon which is located in the human intestine and widespread throughout everywhere in the
world. T. intestinalis was found firstly by Davaine in 1860 and it was called as Cercomonas hominis.
Diagnosis of T. intestinalis can be made by way observing the motile trophozoids in wet mount preparation under
light microscope. In addition to, MS-F (Modified Shaffer-Frye) and CPLM (Cystein-Peptone-Liver-Maltose) media
are suggested for cultivation of this protozoon for diagnosis.
Robinson media that used for amoeba has been tried for cultivation of T. intestinalis
T. intestinalis containing stool samples which were examined under light microscope in the İnonu University Medical
Faculty Department of Parasitology were cultivated in the Robinson media and reproduction was checked every 48
hours. It was observed that the T. intestinalis can survive for 36 days in Robinson media.
As a result, Robinson medium is available for diagnosis of T. intestinalis and trophozoids may be used in educational
research studies also.
Key Words: T. intestinalis, Robinson Medium
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