Background: Ganoderma can live as saprobes on rotting stumps and roots by decomposing lignin as well as cellulose and related polysaccharides. It is because of mushrooms cellulolytic activities. Objective: The aim of this study was to compare the cellulolytic activity of the crude extract of Ganoderma applanatum and G. tropicum from Garut (Indonesia). Method: Colorimetric method using dinitrosalicylic acid (DNS). Results: The cellulolytic activity of G. applanatum and G. tropicum in the pH range of 3.5-10.0 was 0.051-0.109 and 0.063-0.131 UI/mL, respectively. The cellulolytic activity of G. applanatum and G. tropicum in the temperature range of 35-65 °C was 0.100-0.184 and 0.077-0.110 UI/mL, respectively. Conclusion: The activity of cellulase enzymes were affected by temperature and pH. The cellulolytic activity of G. applanatum was the same as G. tropicum.
Key words: cellulolytic, colorimetric, DNS, various pH, various temperature
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