Abstract

Background:
Meat quality and safety are essential issues in the food industry because oxidation and microbial growth directly affect their shelf life and physical and chemical properties. Chitosan and Aloe vera gel are natural compounds known for their antioxidant and antimicrobial properties, making them promising agents for developing food preservation technologies.

Aim:
This study aimed to assess the effects of chitosan nanoparticles (CNPs) and A. vera gel on mutton meat’s physicochemical, microbiological, and oxidative properties during refrigerated storage at 4 °C.

Methods:
Mutton samples were treated in four groups (control, CNPs loaded with A. vera gel, CNPs alone, and A. vera gel alone). They analyzed six aging intervals (0, 1, 2, 5, 8, and 12 days) in a factorial arrangement (4×6). Key chemical parameters, including thiobarbituric acid reactive substances and pH values, as well as microbial counts, particularly the growth of Pseudomonas aeruginosa, were evaluated.

Results:
CNPs loaded with A. vera gel improved sensory characteristics, reduced lipid oxidation, and inhibited P. aeruginosa growth more effectively than other treatments or the control.

Conclusion:
These findings highlight the potential of 2% CNPs loaded with A. vera gel as a natural preservative to enhance refrigerated mutton’s quality and shelf life, which may have promising applications in the food industry.

Key words: Aloe vera, Chitosan nanoparticles, Mutton meat, Pseudomonas aeruginosa, Refrigerator storage