Background:
The Indonesian government aims to encourage the beef cattle population to increase livestock numbers through artificial insemination (AI). However, this initiative encounters challenges due to the low quality of semen following cryopreservation.
Aim:
This study aimed to evaluate the influence of L-arginine supplementation in the Tris egg yolk extender on the motility, kinematic movement, viability, acrosome integrity, and lipid peroxidation of cryopreserved Simmental bull spermatozoa.
Methods:
The experiment involved four groups with 20 replicates each: the control group (CG), Group 1 (G1) using 0.05 M L-arginine, Group 2 (G2) using 0.1 M L-arginine, and Group 3 (G3) using 0.2 M L-arginine in a Tris-Yolk extender. Observations included spermatozoa motility and kinematic movement (CASA), viability, acrosome integrity, and lipid peroxidation measured by MDA (TBA test). Data were analyzed using post hoc analysis of variance, Duncan’s test, with significance p
Key words: Acrosome integrity; Lipid peroxidation; Motility; Tris-egg yolk; Viability.
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