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Therapeutic potential of branched-chain amino acids against carbon tetrachloride-induced cardiopulmonary injuries in Wistar ratsMariam M. Jad, Zeinab Al-Amgad, Fatma A. Madkour, Hend Al-Amgad, Asmaa W. Basher, Heba A. Mohammed, Abeer M. Mahmoud, Asmaa Elnagar, Ibrahim F. Rehan, František Zigo, Martina Zigová, Amany Ahmed Abd El-Aziz. Abstract |  Download PDF | |  Post | Background:
Carbon tetrachloride (CCl4) is a xenobiotic hepatotoxic agent that causes pathophysiological disorders in tissues and the liver. CCl4-induced cardiopulmonary toxicity is attributed to free radical-induced dyslipidemia and oxidative damage. Branched-chain amino acids (BCAAs) stimulate systemic defense against oxidative stress, which is probably caused by CCl4, through an improvement in the antioxidant system.
Aim:
This study aimed to explore the therapeutic benefits of BCAAs against CCl4-induced cardiopulmonary pathologies.
Methods:
A total of 28 inclusive Wistar rats stand under control was standard; group 2 comprised CCl4-received rats, whereas groups 3 and 4 comprised CCl4-rats supplemented with two varying levels of BCAAs.
Results:
Biochemical findings indicated that CCl4 provoked a rise in glucose and cholesterol levels compared with the control. The oxidative profile suggested increased malondialdehyde level and decreased superoxide dismutase activity in the CCl4-intoxicated heart. Supplemented BCAAs downregulated dyslipidemia and restored atrial changes in antioxidant components to be close to normal. Histopathological investigations using hematoxylin-eosin stain revealed damage to the inflamed heart-lung parenchyma of CCl4-intoxicated rats. An increase in fibrosis expression was detectable, simultaneously with a deprivation of protein content in cardiopulmonary sections, as shown by Masson trichrome and bromophenol blue techniques. Co-treated BCAAs intensified cardiopulmonary integrity, inducing scarce histological fibrosis with abundant protein. Accordingly, BCAA supplementation in CCl4-treated rats mitigated complications by reducing serum toxicity, enhancing antioxidant capacity, restoring protein expression, and improving organ health.
Conclusion:
The findings support the protective role of BCAAs with antioxidant benefits against CCl4-induced damage, suggesting their potential as a therapeutic agent for cardiopulmonary pathology.
Key words: CCl4; BCAAs; Dyslipidemia; Antioxidant profile; Cardiac injury.
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