this study was to validate of methods analysis of the α-mangostin, γ-mangostin, and gartanin in mangosteen fruit rind extract derived from Bogor, Purwakarta, Subang and Tasik with High Performance Liquid Chromatography (HPLC) for routine analysis. The methods was employed Enduro C-18 reverse-phase (250 mm x 4.6 mm) column chromatography systems with Photo Diode Array detector 375 nm, and acetonitrile: water containing 0.1% phosphoric acid (95:5) as mobile phase at the rate flow of 1.0 ml/min and validation methods with parameters of linearity, limit of detection and quantification, precision, and accuracy. The analysis showed that the levels of α-mangostin, γ-mangostin, and gartanin from Bogor were 13.87%, 8.28% and 10.44%, respectively. The results from mangosteen pericarps extract from Purwakarta were 10.07% for α-mangostin, 6.33% for γ-mangostin, and 8,.76% gartanin. Mangosteen pericarps extract from Subang concentration of α-mangostin 10.88%, γ-mangostin 6.01%, and gartanin 8.08%. The contains of α-mangostin, γ-mangostin, and gartanin from Tasik, respectively, 8.53%, 6.07%, 17.28%. This study can be concluded that the methods was valid and can be used for routine analysis.
Key words: α-mangostin, γ-mangostin, gartanin, HPLC, Solid Phase Extraction (SPE)
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