Abstract

In our study, innovative Nanoformulation is promising tool to overcome breast cancer. Gold nanoparticles (AuNPs) have gained significant interest for biomedical applications, particularly in diagnostic imaging and therapy. However, it shows some potential nanoparticle toxicity. By making chitosan nanoformula containing nanogold, lipoic, folic acid and doxorubicin. As nanogold has anticancer effect, lipoic acid increase solubility of nanogold and decrease its toxicity additionally folic acid targets nanogold into breast cancer cell as folic acid is on surface of nanoformula and it occurs overexpression of the folic acid receptors on these cells moreover, doxorubicin is anticancer chemotherapy achieving our aim to abolish breast cancer. This is achieved by making four nanofomulas (NC+NG, NC+NG+NF+NL, NC+ND, NC+ND+NF+NL) measuring parameters as cytotoxicity, cell proliferation, migration, colony formation assays. Firstly, We calculated IC50 for NC+NG and NC+ND , Fa values were as following Fa 0.50= 285.93 μg/mL, Fa 0.75= 113.04 μg/mL ,Fa 0.90= 44.68 μg/mL for NC+NG, and were as following Fa50= 42.90 μg/ml, Fa75=15.76 μg/ml, Fa 0.90= 5.79 μg/mL, Fa 0.95= 2.93 μg/mL for NC+ND . we used Fa 75 value of NC+NG for making our assays in both two groups (NC+NG, NC+NG+NF+NL) and used also Fa 75 value of NC+ND for both two groups (NC+ND, NC+ND+NF+NL). All our nanformula significantly caused cytotoxicity compared to control, decreased cell proliferation, inhibited cell migration, reduced colony formation and those formulas directed with folic acid were better than non- directed ones confirming our role in proving targetting influence of folic acid towards over expressed folic acid receptors on MCF7 cells , on conclusion, our nanoformulas were efficient in killing breast cancer cells inhibiting their proliferation, migration and colony formation, so that they are promising tools in destroying cancer cells and solving the problem of breast cancer.

Key words: nanogold, doxorubicin, folic acid, lipoic acid, cytotoxicity, proliferation, migration, colony formation.