Introduction: Nutmeg is well known in Indian medicine for having aromatic, analgesic, antioxidant, antibacterial, antifungal, antidiabetic, hypolipidemic, hepatoprotective, and anti-inflammatory properties. Oleoresin is a natural mixture of resin and essential oil derived from the leaves of the olea europaea tree. Oleoresins are organic solvent-extracted mixtures of essential oils and resins extracted from spices.
Aim: The aim of the current study was to analyse the anticancer activity of zinc oxide nanoparticles synthesized using nutmeg oleoresin on HepG2 Cell line. Materials and method: Nutmeg oleoresin was obtained from synthite industries Ltd,Kerala. MTT assay was performed with nutmeg oleoresin mediated zinc oxide nanoparticles to assess the anticancer activity.The cells were treated with different concentration of nutmeg oleoresin (20,40,60,80,100,200μg/ml) for 24 hours and the cell viability was analysed using MTT assay.
Result and discussion: The results of MTT assay showed a dose dependent increase in anticancer activity of nutmeg oleoresin against the cancer cell line. The IC50 value was 40μg/ml.
Conclusion: The present study within the limitations conclude that nutmeg oleoresin possesses a good level of cytotoxic activity against cancer cell lines. Hence the extract may be further explored in animal models to establish its activity.
Key words: Anticancer, oleoresin, MTT assay, zinc oxide nanoparticle, eco friendly, green synthesis
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