Objective: To study the impact of Wolbachia Surface Protein (WSP) on Reactive oxygen species (ROS) level in Ethanol-exposed HepG2 cells.
Materials and Methods: Increase in ROS level was induced in HepG2 cells by subjecting HepG2 cells to Ethanol exposure. Impact of Wolbachia Surface Protein (WSP) on ROS level was examined by staining of intracellular Reactive oxygen species in cells using the specific ROS-detecting dye 2, 7-dichlorodihydrofluorescein diacetate (H2DCFDA), followed by flow cytometric analysis.
Results and Conclusion: Flow cytometry analysis using H2DCFDA-based staining study of ROS level in HepG2 cells revealed that Ethanol caused Oxidative stress in HepG2 cells by inducing production of high levels of Reactive oxygen species (ROS). However, Ethanol-induced increased ROS production in cells, decreased with treatment of Wolbachia Surface Protein (WSP).
From the current study, we can culminate that Wolbachia Surface Protein (WSP) provides cytoprotective action against Ethanol-induced increased ROS production and Oxidative stress in HepG2 cells by decreasing Reactive oxygen species (ROS) production. This will be of significance for the treatment of Ethanol-related liver ailments. Thus, this article emphasizes that Wolbachia Surface Protein (WSP) with protecting ability could be used as a powerful therapeutic drug to treat Ethanol-related liver ailments.
Key words: Ethanol-related liver ailments, HepG2 cells, Reactive oxygen species (ROS), Therapeutic drug, Wolbachia Surface Protein (WSP)
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