Abstract

Background: African swine fever (ASF) is an important disease affecting swine and has a significant economic loss on both the developed and developing world. Previous studies have indicated the mitigation of African swine fever virus (ASFV) in feed, suggesting that the risk of ASFV transmissibility via the oral exposure.
Aim: We evaluated the potential effects of MCFAs in both individual and synergistic forms, to prevent and/or reduce ASFV infection using in vitro feed model.
Methods: The cytotoxicity of MCFAs on PAM cells were evaluated by using the MTT assay. The potential effects of MCFAs, including C8, C6-C8-C10 (1:1:1 ratio) and C8-C10-C12 (1:1:1 ratio) against a field ASFV strain isolated in the Capital Hanoi of Vietnam were further examined by real-time PCR and HAD assays in in vitro feed model.
Results: Our results indicated that all products do not induce the cytotoxicity at the dose of 100 µg/ml and are suitable for further in vitro examination. All tested products have shown a strong antiviral effect against ASFV infectivity at doses of 0.375% and 0.5%. Interestingly, the synergistic MCFAs have shown clearly their potential activities against ASFV in which at lower dose of 0.25%, pre-treatment with product 2 and 3 induced significant increases at the level of Cq value when compared to positive control and/or product 1 (P

Key words: African swine fever virus; virus isolation; MCFA; pig; Vietnam