Calcium disodium ethylenediaminetetraacetic acid (CaNa2EDTA) has been used for several industrial, nutritional and biomedical applications. This study was conducted to evaluate the possible toxic effect of chronic administration of CaNa2EDTA on male rats. Twenty adult males were divided into the two groups as the following: group 1(n=10) was served as a control and group 2 (n=10) was administered orally by 0.5g/kg of CaNa2 EDTA day after day for three months. At the end of the experiment, blood samples, liver, kidney and testes were collected for hematological, biochemical, antioxidant activity and histopathological investigations. The results showed that upon chronic administration with CaNa2 EDTA, no significant change in body weight of the treated rats, R.B.Cs count, W.B.Cs count, hemoglobin level, hematocrit value and platelets count showed significant increase in treated group. No significant changes in liver enzymes including alanine amino transaminase and aspartate aminotransferase and kidney functions including urea and creatinine. Significant increase in lipid profile including cholesterol, high- and low-density lipoprotein. Significant increase in antioxidant enzymes activity such as superoxide dismutase. No significant change in testosterone hormone were observed between control and treated group. No significant change in serum electrolytes levels including Ca, Na, K and Cl). Histopathologically, mild changes in liver and kidney architectures were noticed. No significant change in testes tissue in the treated group. In summary, chronic administration of CaNa2EDTA for three months did not show substantial toxic effect on male rats.
Key words: Calcium disodium EDTA, chronic toxicity, biochemistry, histology
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